We analysed the Sufu fractions
from the filtration column on a gel. We then pooled the fractions from the GF
column (concentrated and quantitated, aliquoted and flash frozen).
The column was washed
with water and ethanol and we had to change the size of the column for the
second protein which is much smaller in size.
 |
| The Gel Filtration Column |
The Histag purification
was completed with the second protein and samples were taken at each stage and
run on a gel.
Looking at the gels, most of the proteins for
both were found to be insoluble and we decided to
do instead of do can you say run the cultures again but this time incubate them
at 18˚C instead of 37˚C overnight. So we began again, making fresh cultures for
all and a new broth for the giant culture flasks. We decided to go ahead with
the purification of the third protein which we did and incubated overnight
again with rotation.
I keep thinking its Thursday,
we have done so much in the last 2 days that I feel like it should be the end
of the week. I guess the heat doesn’t help, the labs have been incredibly hot
(the weather report lied, they said it would rain, it never did, we need just a
little rain to cool us down).
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