Friday:
I
received an email from Amy saying that there isn’t much to do today and that I
could have the day off – yay for a long weekend! However Amy spent the
day continuing the experiment from yesterday, which was basically
repeating this week's protocol after the initial incubation temperature of
colonies were changed… so all in… straight forward. Amy concentrated the second
protein elute further, she applied in the GF column, same as before, 9
fractions were taken and run on a gel (so also all rather straight forward)
The
Sufu that we had harvested and frozen yesterday was re-suspended in lysis
buffer, sonicated and was purified the same as before, leaving it to be
incubated overnight at 4˚C. (Amy says she wants to come in over the weekend to
finish it off, after which she will also apply to the GF column.)
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